CUSABIO Exosome Isolation Kits purify exosomes by affinity purification. The purity and yield are comparable to ultracentrifugation.
November 13, 2020
In 2019, the team at CUSABIO developed the first commercial Exosome Isolation Kit by affinity purification. Researchers can obtain the High Purity exosomes and other Evs (Extracellular vesicles) from the cell culture medium and body fluid (high yield by normal microfiltration) easily by this method. The exosomes obtained from CUSABIO kits are suitable for many downstream experiments, including Transmission electron microscope analysis, Nanoparticle tracking analysis, NanoFCM analysis, Western Blot, Fluorescence quantitative (qPCR), High-Throughput Sequencing, etc. But it only could extract exosomes from the cell culture supernatant at that time. To meet the requirements of researchers, CUSABIO adds another 5 sample types for the kit in 2020.
About CUSABIO Exosome Isolation Kit
CUSABIO exosome isolation kit can extract exosome from various sample types and cells with high purity and yield. It is suitable for many downstream experiments. This kit is very simple and efficient to use. No ultracentrifugation or phenol/chloroform step required. No large-scale equipment required. And the exosomes extracted by CUSABIO Exosome Isolation Kit are comparable to ultracentrifugation with High Purity and High Yield.
More details, please visit https://www.cusabio.com/Exosome-Isolation-Kit.html
Exosomes are small, single-membrane, secreted extracellular vesicles of ~30 to ~200 nm in diameter that contains any of various biomolecules, such as proteins or nucleic acids. They allow for cell-to-cell communication, transporting molecules, and act as shuttles for certain genetic information and proteins to other cells. Intercellular communication by exosomes plays a critical role in diverse pathological processes, such as cancer, infectious and neurodegenerative diseases.
Exosomes were first discovered in the maturing mammalian reticulocyte by Stahl and group in 1983 and Johnstone and group in 1983, further termed ‘exosomes’ by Johnstone and group in 1987. In 2007, Jan Lotvall and the group made a great breakthrough in exosome research. The variable RNA and miRNA contained in the exosomes, and non-coding long RNAs and mRNAs can be transported between cells. In 2010, three research groups demonstrated that miRNAs can be transferred to standby cells and function in cells.
Since then, the importance of exosome comes out conspicuously. In addition, the Nobel Prize in Physiology or Medicine 2013 was awarded jointly to James E. Rothman, Randy W. Schekman and Thomas C. Südhof “for their discoveries of machinery regulating vesicle traffic, a major transport system in our cells.” More and more researchers start their study of the exosome. And exosomes are being explored as a tool for disease diagnosis and management.
Since exosomes are distributed in a broad range of biofluids, such as serum, plasma, ascites fluid, urine, saliva and tissue culture media, exosome isolation becomesthe first and the most important step in the research of exosome. There are many methods to isolate exosomes from the samples, such as Differential ultracentrifugation and Density gradient ultracentrifugation, Ultrafiltration method, Polypolymer precipitation method, Magnetic bead immunoassay, Aqueous two-phase system (ATPS) method, etc. They have their own pros and cons. Exosome isolation kit is the product that tries to develop the strong points and avoid the weak points. And it turns out the results are not bad. It is also one of the reasons why CUSABIO developed the Exosome isolation kit.
CUSABIO is a manufacturer of ELISA kits, Exosome isolation kits, antibodies, proteins, and related reagents, starting with a small lab. After 13 years of effort, CUSABIO has become a good partner to researchers worldwide. And thousands of products are used by the researchers and help them make more and more breakthroughs.
To know more about CUSABIO, please visit: https://www.cusabio.com/
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