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N-Glycan Characterization via LC-MS, Upcoming Webinar Hosted by Xtalks

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Xtalks Life Science Webinars

N-glycosylation is a post-translational modification (PTM) that plays a crucial role in monoclonal antibody (mAb) effector functions and is, therefore, classified as a critical quality attribute (CQA) that requires comprehensive, precise and accurate characterization and monitoring.


TORONTO (PRWEB)

Join James Duffy, Assay Development Scientist, BioPharm CMC, Covance Inc. in a live webinar on Thursday, September 17, 2020 at 11am EDT (4pm BST) in which he will discuss the LC-MS approach to N-glycan characterization for a variety of biopharmaceutical products, as well as best practices for overcoming some of the most common product-related challenges.

N-glycosylation is a post-translational modification (PTM) that plays a crucial role in monoclonal antibody (mAb) effector functions and is, therefore, classified as a critical quality attribute (CQA) that requires comprehensive, precise and accurate characterization and monitoring.

N-glycosylation of mAbs (and other therapeutic proteins) has the potential to influence the pharmacodynamics (PD) and pharmacokinetic (PK) behavior, overall therapeutic efficacy and safety of the product because certain N-glycan species can cause adverse immune reactions, even at very low abundances. Therefore, a highly sensitive assay that can identify and quantify glycan species below 0.1 percent relative abundance is essential.

The webinar will provide:


  • An introduction to glycosylation, typically observed N-glycan species and their importance as CQAs

  • An overview of platform LC-MS workflows for ‘first-look’ N-glycan profiling including intact mass, middle-up and peptide mapping analyses

  • In-depth review of our current released N-glycan method using RapiFluor-MSTM labelling, with comparison to the traditional 2-AB technique

  • An overview of the supplementary exoglycosidase linkage analysis used to provide additional confidence in N-glycan assignments and differentiate between isobaric species

  • Focus on fusion proteins and products with additional N-glycan sites outside of the Fc-region with example data from our Aflibercept AMF, highlighting the challenges associated with analyzing more complex N-glycan profiles, as well as an overview of a specific digestion and separation strategy for assigning N-glycan profiles to discrete regions of the protein and further use of peptide map LC-MS analysis to identify site-specific glycan occupancy

For more information or to register for this event, visit N-Glycan Characterization via LC-MS.

ABOUT XTALKS

Xtalks, powered by Honeycomb Worldwide Inc., is a leading provider of educational webinars to the global life science, food and medical device community. Every year thousands of industry practitioners (from life science, food and medical device companies, private & academic research institutions, healthcare centers, etc.) turn to Xtalks for access to quality content. Xtalks helps Life Science professionals stay current with industry developments, trends and regulations. Xtalks webinars also provide perspectives on key issues from top industry thought leaders and service providers.

To learn more about Xtalks visit http://xtalks.com

For information about hosting a webinar visit http://xtalks.com/why-host-a-webinar/

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