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A More Effective Purification of Therapeutic Peptides Using Orthogonal Techniques, Upcoming Webinar Hosted by Xtalks

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Reversed phase chromatography (RPC) is the most commonly used peptide separation technique but has its challenges. Impurities from the peptide feed can cause fouling of the column and not all impurities can


TORONTO (PRWEB)

Regardless of the peptide production method — peptide synthesis or expression — in host cells, all crude peptides need to be purified. The purity requirements for therapeutic peptides are particularly stringent. Reversed phase chromatography (RPC) is the most commonly used peptide separation technique but has its challenges. Impurities from the peptide feed can cause fouling of the column and not all impurities can be resolved on the RPC column. Adding an orthogonal step to both protect the RPC column and increase the resolution, could solve these issues. For example, a variety of peptide modifications that cannot be removed by RPC can be separated by ion exchange chromatography. By introducing a cation exchange chromatography (CIEX) step upstream of the high-performance silica-based RPC step, the burden from impurities is significantly reduced and the purity of the target peptide is greatly enhanced.

In this webinar, the featured speaker will present data on three differently produced therapeutic peptides: two solid-phase synthesized peptides and one peptide expressed in Escherichia coli, both purified with an upstream CIEX step before one or two RPC steps. Two of the cases presented are directed more towards process scale and one is specifically directed against screening and small-scale purifications.

Register for this webinar to hear about the orthogonal chromatography techniques used in the purification of therapeutic peptides.

Join Cecilia Unoson, Manager Applications, Bio-Works, for the live webinar on Thursday, May 19, 2022, at 10am EDT (3pm BST/UK).

For more information, or to register for this event, visit A More Effective Purification of Therapeutic Peptides Using Orthogonal Techniques.

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