The tag not only helps to yield highly pure proteins, but also provides the option to detect a specific protein of interest via ELISA (enzyme-linked immunosorbent assay) or western blot.
TORONTO (PRWEB)
September 22, 2022
The identification and production of monoclonal antibodies is important for investigating immune responses and developing therapies. The entire process involves many steps including antigen expression, immunizing host species, isolating antigen-specific B cells as well as clone screening for detecting high affinity antibodies. A protein tag can provide options to centralize various steps onto one platform, making the entire process more time- and cost-effective.
IBA Lifesciences’s Strep-tag® system — a protein tag system based on the interaction between a synthetic peptide and specially engineered streptavidin proteins — offers multiple choices of interaction partners from micromolar to picomolar affinity. As a result of the differences in binding strength among the possible tag-ligand combinations, this system has become a universal tool for isolation of proteins, cells and exosomes. The tag not only helps to yield highly pure proteins, but also provides the option to detect a specific protein of interest via ELISA (enzyme-linked immunosorbent assay) or western blot.
Due to the binding to high affinity Strep-Tactin®XT (an engineered streptavidin) in the picomolar range, even biosensor applications such as surface plasmon resonance (SPR) or bio-layer interferometry (BLI) are possible. In addition, incubating the Strep-tagged protein with another engineered streptavidin conjugate generates reagents suitable for cell staining and isolation. This highlights the possibilities that this technology offers beyond protein research.
Join this webinar to learn how the Strep-tag® protein tag system not only enables protein purification, but also further analyses such as affinity measurements, ELISA, western blot as well as staining and isolating cells, making it a useful tool for complex processes such as antibody screening and production.
Join Fabian Mohr, Vice President Research & Development, IBA Lifesciences, for the live webinar on Wednesday, October 12, 2022, at 1pm EDT (10am PDT).
For more information, or to register for this event, visit How a Single Protein Tag Provides a Platform for Key Processes in Antibody Production.
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